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13 November 2020

bromophenol blue dye

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Bromophenol blue is also used as a pH indicator, with a transition range of pH 3 to 4.6. Bromophenol blue is also used as a pH indicator, with a transition range of pH 3 to 4.6. At low pH, the dye absorbs ultraviolet and blue light most strongly and appears yellow in solution. Guaranteed product quality, expert customer support. All rights reserved. If you continue without changing your cookie settings, we'll assume you’re happy with this. Solutions of the dye, therefore, are blue. Tracking dye in DNA, RNA (agarose) and protein (polyacrylamide) gel electrophoresis. Please let us know so that we can cite the reference in this datasheet. All of the co-solvents are provided by MCE. Based on 1 publication(s) in Google Scholar. Bromphenol Blue - Compound Summary. Thanks, your subscription has been confirmed. It has also been used as an industrial d Sorry, but the email address you supplied was invalid. * Please select Quantity before adding items. We will send it to your Email address shortly. Bromophenol blue (115-39-9) Chemical Names and Synonyms. https://www.scbt.com/scbt/product/bromophenol-blue-115-39-9, https://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi?cid=8272&loc=ec_rcs, http://www.sigmaaldrich.com/Graphics/COfAInfo/SigmaSAPQM/SPEC/B0/B0126/B0126-BULK________SIAL_____.pdf, "Agarose gel electrophoresis (basic method)", "Quantification of dichromatism: a characteristic of color in transparent materials", https://en.wikipedia.org/w/index.php?title=Bromophenol_blue&oldid=984535214, Pages using collapsible list with both background and text-align in titlestyle, Articles containing unverified chemical infoboxes, Creative Commons Attribution-ShareAlike License, This page was last edited on 20 October 2020, at 16:16. PubChem Compound. In solution at pH 3.6 (in the middle of the transition range of this pH indicator) obtained by dissolution in water without any pH adjustment, bromophenol blue has a characteristic green red colour, where the apparent colour shifts depending on … We use cookies to make our site as useful as possible. [5] Solutions of the dye, therefore, are blue. Reaxys. Bromophenol blue is also used as a pH indicator, with a transition range of pH 3 to 4.6[1][2]. For research use only. We're doing our best to keep everyone healthy and safe in the workplace while also avoiding the interruptions to our day-to-day operations. At neutral pH, the dye absorbs red light most strongly and transmits blue light. Tracking dye for nucleic acid or protein electrophoresis. It is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. Products are chemical reagents for research use only and are not intended for human use. 2019 Mar 22; 540: 148-154. Your need for high quality reagent doesn't stop during difficult times, and neither do we. Need more advice on solubility, usage and handling? Bromophenol blue is also used as a dye. You will hear from us soon. Bromophenol blue is an acid phthalein dye, and it is used as a tracking dye for electrophoresis. For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome. Please refer to the solubility information to select the appropriate solvent. [1]. To our knowledge, customised protocols are not required for this product. Bromophenol blue (3′,3″,5′,5″-tetrabromophenolsulfonphthalein, BPB,[2] albutest[3]) is used as a pH indicator, an electrophoretic color marker, and a dye. [2]. Preferential solvation bromophenol blue in water-alcohol binary mixture. . Store under desiccating conditions. 2019 Mar 22; 540: 148-154. Get resources and offers direct to your inbox. Bromophenol blue is 3H-2,1-Benzoxathiole 1,1-dioxide in which both of the hydrogens at position 3 have been substituted by 3,5-dibromo-4-hydroxyphenyl groups. Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery. Please note: All products are "FOR RESEARCH USE ONLY. Nanocellulose for gel electrophoresis. Room temperature in continental US; may vary elsewhere. Please contact us to place your order, or try again later. If incorrect, please enter your country/region into the box below, to view site information related to your country/region. J Colloid Interface Sci. © 1998-2020 Abcam plc. Dangui AZ, et, al. Please store the product under the recommended conditions in the Certificate of Analysis. Bromophenol blue has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. Spectrochim Acta A Mol Biomol Spectrosc. Please fill out this form to request the QC report. DMSO : 50 mg/mL (74.63 mM; Need ultrasonic), Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline, Solubility: ≥ 2.5 mg/mL (3.73 mM); Clear solution, Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline). [8] This means it has the largest change in colour hue, when the thickness or concentration of observed sample increases or decreases. Generally, these will be useable for up to one month. Migrates at approximately the same rate as 300-500bp DNA… Bromophenol blue, Tracking dye (CAS 115-39-9) (ab146339) | Abcam Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. The product can be stored for up to 12 months. In solution at pH 3.6 (in the middle of the transition range of this pH indicator) obtained by dissolution in water without any pH adjustment, bromophenol blue has a characteristic green red colour, where the apparent colour shifts depending on the concentration and/or path length through which the solution is observed. It can be prepared by slowly adding excess bromine to a hot solution of phenolsulfonphthalein in glacial acetic acid.[4]. If you need to change the delivery plan for items ordered, please contact us via email [email protected]. Santa Cruz Biotechnology, Inc. Bromophenol Blue (CAS 115-39-9) Properties. Mendoza L, et, al. Store at Room Temperature. Tracking dye in DNA, RNA (agarose) and protein (polyacrylamide) gel electrophoresis. Preferential solvation bromophenol blue in water-alcohol binary mixture. If you have published this work, please enter the PubMed ID. At neutral pH, the dye absorbs red light most strongly and transmits blue light. There are currently no Customer reviews or Questions for ab146339.Please use the links above to contact us or submit feedback about this product. It changes from yellow at pH 3.0 to blue at pH 4.6; this reaction is reversible. As the situation with COVID-19 continues to unfold in every community, MedChemExpress is responding to the uncertainty caused by this outbreak thoughtfully and cautiously. Your browser does not have JavaScript enabled and some parts of this website will not work without it. We have received your request and will respond to you as soon as possible. Our Cookie Policy explains how you can opt-out of the cookies we use. Publishing research using ab146339? InChI=1S/C19H10Br4O5S/c20-12-5-9(6-13(21)17(12)24)19(10-7-14(22)18(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28-19/h1-8,24-25H, InChI=1/C19H10Br4O5S/c20-12-5-9(6-13(21)17(12)24)19(10-7-14(22)18(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28-19/h1-8,24-25H, Brc1cc(cc(Br)c1O)C3(OS(=O)(=O)c2ccccc23)c4cc(Br)c(O)c(Br)c4, Except where otherwise noted, data are given for materials in their. It is used as a laboratory indicator, changing from yellow below pH 3 to purple at pH 4.6, and as a size marker for monitoring the progress of agarose gel and polyacrylamide gel electrophoresis. Bromophenol blue is also used as a dye. As an acid–base indicator, its useful range lies between pH 3.0 and 4.6. Wherever possible, you should prepare and use solutions on the same day. Bromphenol Blue - Use and Manufacturing. Spectrochim Acta A Mol Biomol Spectrosc. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C.

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